Reporter
Part:BBa_K3826005:Design
Designed by: Lucas M�ller Group: iGEM21_Duesseldorf (2021-10-21)
mVenus yellow fluorescent protein, Gert-Jan Kremers et al. (https://pubs.acs.org/doi/10.1021/bi05162
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 630
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 28
Illegal AgeI site found at 151 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
2 segments
translation: MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
239 amino acids = 26,9 kDa
Source
The source of the protein is the jellyfish Aequorea victoria.